Treponema pallidum p15, Recombinant (Syphilis)

Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames.\n\nThe protein contains the Trp. Pallidum p15 immunodominant regions.\n\nSpecificity:\nImmunoreactive with sera of Trp. Pallidum infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blot, excellent antigen for detection of Trp. Pallidum with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8300-17A

Size

100ug

Form

Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA, 1mM DTT, 8M urea.

Purity

(same/more than) 90% by 10% PAGE (coomassie staining)

References

1. Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses. Centurion-Lara A, Arroll T, Shaffer JM, Infect Immun 1997 Apr;65(4):1440-4 2. Epitope mapping of B-cell determinants on the 15-kilodalton lipoprotein of Treponema pallidum (Tpp15) with synthetic peptides. Baughn RE, Demecs M, Musher DM, Infect Immun 1996 Jul;64(7):2457-66 3. Effects of cefetamet (Ro 15-8074) on Treponema pallidum and experimental syphilis. Fitzgerald TJ, Antimicrob Agents Chemother 1992 Mar;36(3):598-602 4. Lipid modification of the 15 kiloDalton major membrane immunogen of Treponema pallidum. Purcell BK, Swancutt MA, Mol Microbiol 1990 Aug;4(8):1371-9 5. Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum. Purcell BK, Chamberlain NR, Andrews LP, Infect Immun 1989 Dec;57(12):3708-14.