Treponema pallidum p47, Recombinant (Syphilis)

Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames.\n\nThe protein contains the Trp. Pallidum p47 immunodominant regions.\n\nSpecificity:\nImmunoreactive with sera of Trp. Pallidum infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blot, excellent antigen for detection of Trp. Pallidum with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8300-19A

Size

100ug

Form

Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA, 1mM DTT, 8M urea.

Purity

(same/more than) 90% by 10% PAGE (coomassie staining)

References

1. Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein. Deka RK, Machius M, Tomchick DR, J Biol Chem 2002 Nov 1;277(44):41857-64 2. Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells. Lee KH, Choi HJ, Lee JB, Yonsei Med J 2000 Oct;41(5):623-33 3. Molecular mimicry between an immunodominant amino acid motif on the 47-kDa lipoprotein of Treponema pallidum (Tpp47) and multiple repeats of analogous sequences in fibronectin. Baughn RE, Jiang A, Ottmers V, J Immunol 1996 Jul 15;157(2):720-31 4. The 47-kDa major lipoprotein immunogen of Treponema pallidum is a penicillin-binding protein with carboxypeptidase activity. Weigel LM, Radolf JD, Proc Natl Acad Sci U S A 1994 Nov 22;91(24):11611-5 5. Analysis of the N-terminal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum. Weigel LM, Brandt ME, Infect Immun 1992 Apr;60(4):1568-76 6. Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity. Chamberlain NR, DeOgny L, Radolf JD, Infect Immun 1989 Sep;57(9):2878-85.