Home  >  Products  >  TRIAD3A (Triad Domain-containing Protein 3, TRIAD3, E3 Ubiquitin-protein Ligase RNF216, RING Finger Protein 216, Rnf216, UbcM4-interacting Protein 83, UIP83, Ubiquitin-conjugating Enzyme 7-interacting Protein 1, Ubce7ip1, Zin)
TRIAD3A (Triad Domain-containing Protein 3, TRIAD3, E3 Ubiquitin-protein Ligase RNF216, RING Finger Protein 216, Rnf216, UbcM4-interacting Protein 83, UIP83, Ubiquitin-conjugating Enzyme 7-interacting Protein 1, Ubce7ip1, Zin)

TRIAD3A (Triad Domain-containing Protein 3, TRIAD3, E3 Ubiquitin-protein Ligase RNF216, RING Finger Protein 216, Rnf216, UbcM4-interacting Protein 83, UIP83, Ubiquitin-conjugating Enzyme 7-interacting Protein 1, Ubce7ip1, Zin)

Cat no: T8319-05D

Supplier: United States Biological
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Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubiquitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse heart cell lysates. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8319-05D
Reactivities: Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: 2810055G22Rik, AI647468, AU019462, C86502, EC=6.3.2.-, F830018F18Rik
References: 1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 2. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 3. Sweet MJ, Leung BP, Kang D, et al. A novel pathway regulating lipopolysaccharide-induced shock by ST2/T1 via inhibition of Toll-like receptor 4 expression. J. Immunol. 2001; 166:6633-9. 4. Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7. 5. Chuang T-H and Ulevitch, RJ. Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. Nat. Immunol. 2004; 5: 495-502.
Additional info: Recognizes mouse TRIAD3A.

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