Trichinella, BioAssay(TM) ELISA Kit

For the qualitative screening of serum IgG antibodies to Trichinella using an ELISA technique.\n\nBackground:\nTrichinosis, the infection caused by the nematode Trichinella spiralis, is acquired by ingestion of raw or undercooked meats (primarily pork). Although the nematode may be found in a wide variety of animals worldwide, the domestic pig is the primary source of infection in developed nations. Serology has also been an important tool in the diagnosis of trichinosis for several decades. Various methodologies; such as ELISA, latex agglutination (LA), indirect hemagglutination (IHA) and bentonite flocculation (BFT) have been used. Although various classes of antibodies have been detected, no single class has shown superior diagnostic ability over the others. BFT has been the method of choice for serology but suffers from nonspecific reactions, some lack of sensitivity (measurable antibodies often do not appear until 3 to 4 weeks after infection) and difficulty in performing the test. Recently, an excretory-secretory (ES) antigen has been purified from the larvae of infected pigs. This antigen has a high degree of specificity for T. spiralis and has been used in several large scale studies.\n \nKit Components:\n1. Microtiter Plate (Trichinella antigens): 1x96wells\n2. Protein A (HRP): 1x11ml \n3. Positive Control: 1x2ml \n4. Negative Control: 1x2ml \n5. Tetramethylbenzidine (TMB): 1x11ml\n6. Wash Buffer, 20X: 1x25ml\n7. Dilution Buffer: 2x30ml \n8. Stop Solution: 1x11ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8342

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Despommier, D.: Trichinellosis. Immunodiagnosis of Parasitic Diseases, Vol. 1, Helminthic Diseases. Ed. Walls and Schantz. Academic Press, 1986. pp. 163-181. 2. Krogstad D., et al., Manual of Clinical Microbiology, 4th Ed.. American Society for Microbiology, Washington, DC. 1985, pp. 654. 3. Kagan, I.: Serodiagnosis of Parasitic Diseases. Manual of Clinical Laboratory Immunology. 3rd Ed.. American Society for Microbiology, Washington, DC. 1986, p. 474-477. 4. Petri, W., et.al.: Common-Source Outbreak of Trichinosis Associated With Eating Raw Home-Butchered Pork. So Medical J, August 1988, pp. 1056-1058. 5. Oliver, D., et.al.: Enzyme Linked Immunoassay For Detection of Trichinosis In Humans. Proc 7th Int Conf on Trich, Oct. 1988. 6. Ivanoska, D., et.al.: Comparative Efficacy of Antigen and Antibody Detection Tests for Human Trichinellosis. J Parasit, 75: 1, 1989, pp. 38-41.