Triglyceride Assay Kit, BioAssay(TM)

Triglyceride, also known as Triacylgtriglyceride or Triacyl-glyceride, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. \n\nSimple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery. Triglyceride assay uses a single Working Reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample.\n\nKey Features:\nSensitive and Accurate: Use as little as 10uL of samples. \nLinear Detection Range: 0.01-1.0mMol/L (0.88-88.5 mg/dL) triglyceride.\nSimple and Convenient: The procedure involves addition of a single working reagent and incubation for 30 min at RT, compatible with high throughput assays.\nImproved Reagent Stability: The optimized formulation has greatly enhanced the reagent and signal stability.\n\nApplications:\nDirect Assays: Triglyceride in biological samples (e.g. serum and plasma)\nDrug Discovery/Pharmacology: Effects of drugs on triglyceride metabolism\n\nKit Compnents:\n*T8417-01A: Assay Buffer, 1x24ml. Supplied as a liquid in Potassium phosphate and Magenesium acetate\nT8417-01B: ATP (Adenosine 5?-triphosphate), 1x250ul \nT8417-01C: Dye Reagent (10-Acetyl-3,7-dihydroxyphenoxazine), 1x220ul\nT8417-01D: Enzyme Mix, 1x500ul \nT8417-01E: Lipase, 1x1ml\nT8417-01F: Standard (10mMol/L), 1x100ul. Supllied as a powder with glycerol\n\nStorage and Stability: \nStore *T8417-01A at 4 degrees C and other reagents at -20 degrees C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8417-01

Size

1Kit

References

1. Kim HS et al (2010). Hepatic-specific disruption of SIRT6 in mice results in fatty liver formation due to enhanced glycolysis and triglyceride synthesis. Cell Metab. 12(3): 224-36. 2. Tam J et al (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8): 2953-66. 3. Guo H et al (2011). Anthocyanin inhibits high glucose-induced hepatic mtGPAT1 activation and prevents fatty acid synthesis through PKCZ. J Lipid Res. 52(5): 908-22.