Triiodothyronine, Human, BioAssay(TM) ELISA Kit (T3)

Triiodothyronine, Human, BioAssay(TM) ELISA Kit is for the quantitative measurement of total triiodothyronine in human serum. \n\nBackground:\nThe human thyroid gland is a major component of the endocrine system. Thyroid hormones perform many important functions. They exert powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance of the body, as well as on the maintenance of metabolic activity and the development of the skeletal and organ system. The hormones thyroxine (T4) and 3,5,3' thiiodothyronine (T3) circulate in the bloodstream, mostly bound to the plasma protein, thyroxine binding globulin (TBG). The concentration of T3 is much less than that of T-4, but its metabolic potency is much greater. T3 determination is an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 values and normal T4 values. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated patients. The clinical significance of T3 is also evident in patients in whom euthyroidism is attributable only to normal T3, although their T4 values are subnormal. T3 determination is also useful in monitoring both patients under treatment for hyperthroidism and patients who havediscontinued anti-thyroid drug therapy. It is especially valuable in distinguishing between euthyroid and hyperthyroid subjects. In addition to hyperthyroidism, T3 levels are elevated in women who are pregnant, and in women receiving oral contraceptives or estrogen treatment, paralleling TBG increases in a manner analogous to T4 levels. Likewise, a reduction in TBG concentration decreases T3 concentration. These changes in the T3 level, however, are not a true reflection of thyroid status.\n\nKit Components:\n1. Microtiter Plate: 1x96 wells \n2. T3 (HRP) Concentrate: 1x0.8ml \n3. T3 (HRP) Diluent: 1x15ml \n4. Reference Standard: 1 set \n5. Tetramethylbenzidine (TMB): 1x12ml\n6. Stop Solution: 1x12ml\n7. Wash Buffer, 50X: 1x15ml \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8425-14

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Walker W.H.C. Introduction: An Approach to Immunoassay. Clin. Chem. 1977; 23: 384 2. Kirkegaard C., Friis T. and Siersback-Nielsen K. Acta Endocrinol. 1974; 77: 71 3. Wisdom G.B. Enzyme-Immunoassay. Clin. Chem. 1976; 22: 1243 4. Hoffenberg R. Medicine 1978; 8: 392 5. Lieblich J., Utiger R.D. J. Clin. Invest. 1972; 51: 1939.