TRIM25, NT (E3 Ubiquitin/ISG15 Ligase TRIM25, Estrogen-responsive Finger Protein, EFP, RNF147, ZNF147, RING Finger Protein 147, Tripartite Motif-containing Protein 25, Ubiquitin/ISG15-conjugating Enzyme TRIM25, Zinc Finger Protein 147)

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Thymus Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146201

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA 1993; 11117-20. 2. Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA 1999; 96:12027-32. 3. Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer 2001; 91:152-8. 4. Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature 2002; 417:871-5.

Additional Info

Recognizes human TRIM25.