TRIP15 (Thyroid Receptor Interacting Protein 15, SGN2, ALIEN, COPS2, COP9 constitutive photomorphogenic homolog subunit 2, thyroid receptor interacting protein 15)

Applications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nPeptide ELISA: 1:16,000. \nWestern Blot: 1-3ug/ml. A band is detected at 48kD in Human Brain, Mouse Brain lysates and in lysates of Mouse putuitary cell line NIH3T3 (calculated MW of 51.6kDa according to NP_004227.1). \nImmunohistochemistry: 3-5ug/ml In paraffin embedded Human Thyroid Gland shows nuclear and cytoplasm staining in activated epithelial cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See T8493-02C-P. Antibody

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SPECIFICATIONS

Catalog Number

T8493-02C

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

References

Escher N, Kob R, Tenbaum SP, Eisold M, Baniahmad A, von Eggeling F, Melle C.\nVarious members of the E2F transcription factor family interact in vivo with the\ncorepressor alien.. J Proteome Res. 2007 Mar;6(3):1158-64.

Additional Info

Recognizes human TRIP15. Species Crossreactivity: mouse. Species sequence homology: rat, canine and bovine.

Alternative Names

TRIP15; thyroid receptor interacting protein 15; SGN2; ALIEN; COPS2; COP9 constitutive photomorphogenic homolog subunit 2; thyroid receptor interacting protein 15; COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis); COP9, subunit 2