Trk (Tyrosine Kinase Receptor)

Trk is a family of neurotrophin receptors associated with both the central and peripheral nervous systems. It was first identified as an oncogene in human colon carcinoma, however, there are now three known members of the trk family: trkA, trkB, and trkC. Neurotrophins mediate signaling in neurons by activating trk. NGF, BDNF and NT4/5, and NT-3 are the preferred ligands for trkA, trkB, and trkC, respectively.\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunoprecipitation: 2-5ug/mg \nImmunohistochemistry: Paraffin sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8662-51

Size

100ug

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified from ascites raised in Balb/c mice.

Isotype

IgG1

References

1. Muragaki, Y., et al., J. Comparative Neurology (1995, in press). 2. Martin-Zanca, D., et al., Nature 319: 743-748, 1986. 3. Cordon-Cardo, C., et al., Cell 66: 173-183, 1991. 4. Ip, N.Y., et al., Neuron 10: 1993. 5. Rao, M.S., et al., Proc. Natl. Acad. Sci. USA 95: 3,996-4,001, 1998.

Additional Info

Recognizes human trkA, trkB and trkC. Reactivity has also been seen with transfected rat trkA, trkB and trkC. Reactivity has been shown with glycosylated (gp140trk), partially glycosylated (gp110 trk) and unglycosylated (gp80trk) trk.