Trk (Tyrosine Kinase Receptor)

The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein Trk that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments indicate that Trk binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Nerve growth factor and its high-affinity receptor Trk are thought to be involved in the progression of various cancers (2). NGF interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and Trk. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving Trk and the low-affinity NGF receptor (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-1:20,000 \nFlow Cytometry: 1:60\nImmunoprecipitation: 1:20\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8662-52

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Fujimoto M, et al. Oncogene 28(32): 5108-18, 2005 2. Hempstead BL, et al. Nature 350(6320):678-83, 1991 3. Kaplan DR, et al. Science 252(5005):554-8, 1991.

Additional Info

Recognizes human Trk phosphorylated and unphosphorylated Trk. Species crossreactivity: mouse and rat.

Alternative Names

Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, p140-TrkA, Trk-A\n