The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein Trk that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments indicate that Trk binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Nerve growth factor and its high-affinity receptor Trk are thought to be involved in the progression of various cancers (2). NGF interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and Trk. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving Trk and the low-affinity NGF receptor (3).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:10,000-1:20,000
Flow Cytometry: 1:60
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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