TrkA, TrkB, phosphorylated, TrkA (Tyr490), TrkB (Tyr516) (Tyrosine Kinase Receptor A,B)

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the\nsequence of these family members is highly conserved, these family members are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4 and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1).\nPhosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation\nat this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements chimera) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies (breast, colon, prostate and thyroid carcinomas and acute myeloid\nleukemia) have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the\nneural crest (1).\n\nThe phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence (7). TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. In neuroblastomas overexpression of TrkB correlates with unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by\nadditional overexpression of brain-derived neurotrophic factor BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms

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SPECIFICATIONS

Catalog Number

T8663-15B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol..

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Pierotti, M.A. and Greco, A. (2006) Cancer Lett. 232, 90-98. 2. Segal, R.A. and Greenberg, M.E. (1996) Annu. Rev. Neurosci. 19, 463-489. 3. Stephens, R.M. et al. (1994) Neuron 12, 691-705. 4. Obermeier, A. et al. (1993) EMBO J. 12, 933-941. 5. Obermeier, A. et al. (1994) EMBO J. 13, 1585-1590. 6. Yao, R. and Cooper, G.M. (1995) Science 267, 2003-2006.

Additional Info

Recognizes endogenous levels of human TrkA and TrkB at 140kD only when phosphorylated at Tyr490 and Tyr516, respectively. This antibody may cross-react with Bcr-Abl phosphorylated at an unkown tyrosine residue. Species Crossreactivity: rat. Species sequence homology: mouse.