TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

The Trk proto-oncogene encodes a 140kDa, membrane- spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10e-9M. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2500-1:5000 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:100-1:250 \nFlow Cytometry: 1:30 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8663-09

Size

100ul

Applications

ELISA, FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kaplan, D.R., et al. Science 252:554-558, (1991). 2. Indo, Y., et al, Jpn J Hum Genet 42:343-351 (1997). 3. Gimm, O. et al. J Clin Endocrin Metab 84:2784-2787 (1999).

Additional Info

Recognizes both phosphorylated and unphosphorylated human Trk A. Species crossrectivity: mouse and rat.

Alternative Names

Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, p140-TrkA, Trk-A