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TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Cat no: T8663-09

Supplier: United States Biological
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The Trk proto-oncogene encodes a 140kDa, membrane- spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10e-9M. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2500-1:5000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Flow Cytometry: 1:30 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: T8663-09
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, p140-TrkA, Trk-A
References: 1. Kaplan, D.R., et al. Science 252:554-558, (1991). 2. Indo, Y., et al, Jpn J Hum Genet 42:343-351 (1997). 3. Gimm, O. et al. J Clin Endocrin Metab 84:2784-2787 (1999).
Additional info: Recognizes both phosphorylated and unphosphorylated human Trk A. Species crossrectivity: mouse and rat.

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