Trophoblast Protein

Trophoblast Protein demonstrates a striking and consistent immunohistological reactivity around sites of trophoblastic invasion in the human placenta. Despite preliminary data suggesting that this antibody recognizes hyaluronic acid, it has not been possible to confirm the precise antigenic reactivity of this reagent. Although the immunohistological staining of placenta is abolished by treatment with hyaluronidase, it has not been possible to demonstrate binding of the antibody to purified hyaluronic acid.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: Neat-1:10\nImmunohistochemistry (frozen): Neat-1:10 \nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nPS-NS1/1-Ag4.1 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T6450

Size

2ml

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum, NHP

Form

Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgM

References

1. Sunderland, C. A. et al. (1981) Monoclonal antibodies to human syncytiotrophoblast. Immunology 43: 541-546. 2. Sunderland, C. A. et al. (1985) Immunohistological and biochemical evidence for a role for hyaluronic acid in the growth and development of the placenta. J. Reprod. Immunol. 8: 197-212. 3. Comper, W. D. & Laurent, J. C. (1978) Physiological function of connective tissue polysaccharides. Physiol. Rev. 58: 255-315. 4. Cidadao, A. J. et al. (1990) Immunocytochemical study of tissue distribution and hormonal control of chondroitin -, dermatan - and keratan sulfates from rodent uterus. Eur. J. Cell Biol. 52: 105-116

Additional Info

Recognizes human Trophoblast protein. Species Crossreactivity: Cynomolgus monkey. Does not react with rat.