Trophoblast Protein

Cat no: T6450

Supplier: United States Biological

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Trophoblast Protein demonstrates a striking and consistent immunohistological reactivity around sites of trophoblastic invasion in the human placenta. Despite preliminary data suggesting that this antibody recognizes hyaluronic acid, it has not been possible to confirm the precise antigenic reactivity of this reagent. Although the immunohistological staining of placenta is abolished by treatment with hyaluronidase, it has not been possible to demonstrate binding of the antibody to purified hyaluronic acid. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10 Immunohistochemistry (frozen): Neat-1:10 Optimal dilutions to be determined by the researcher. Hybridoma: PS-NS1/1-Ag4.1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	T6450
Reactivities:	Human, Non-Human Primate
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry
Size:	2ml
Form:	Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
P type:	Mab
Isotype:	IgM
Purity:	Supernatant
References:	1. Sunderland, C. A. et al. (1981) Monoclonal antibodies to human syncytiotrophoblast. Immunology 43: 541-546. 2. Sunderland, C. A. et al. (1985) Immunohistological and biochemical evidence for a role for hyaluronic acid in the growth and development of the placenta. J. Reprod. Immunol. 8: 197-212. 3. Comper, W. D. & Laurent, J. C. (1978) Physiological function of connective tissue polysaccharides. Physiol. Rev. 58: 255-315. 4. Cidadao, A. J. et al. (1990) Immunocytochemical study of tissue distribution and hormonal control of chondroitin -, dermatan - and keratan sulfates from rodent uterus. Eur. J. Cell Biol. 52: 105-116
Additional info:	Recognizes human Trophoblast protein. Species Crossreactivity: Cynomolgus monkey. Does not react with rat.

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Trophoblast Protein

Cat no: T6450

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