Tropomyosin 9a (TPM9a, TM9a)

Tropomyosin (TM) is widely distributed in cell types associated with actin as a fibrous molecule. It is polymerized end to end along each of the two grooves of the F-actin filament providing structural stability and modulating the filament function. To accommodate the wide range of functions associated with actin filaments, TM exists in a large number isoforms. These isoforms, which are expressed by alternative promoters and alternative RNA processing of four genes, TPM1, 2, 3 and 4, consist of six or seven regions of about 40 residues that are considered to represent the actin-binding regions of the molecule. A large part of the polypeptide chains of the TM isoforms, mainly centrally located and expressed by five exons, is invariant. Many of the isoforms are tissue- and filament-specific suggestingg that the variant exons are significant for the function of the filament system in a particular cell type. In the case of muscle there is clear evidence that the TM moves its position on the F-actin filament during contraction. It is unclear how TM in muscle compares to that in non-muscle systems and if its function in muscle is unique. [modified from Perry, 2001] \n\nApplications: \nSuitable for use in Western Blotting, Immunocytochemistry, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:500.\nImmunocytochemistry: 1:50-1:500\nImmunohistochemistry: 1: 1:50-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8664-70

Size

100ul

Applications

ICC, IHC, WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Vrhovski, B., et al., Localization of tropomyosin isoforms during mouse development and the establishment of isoform specific knockouts. Australian Society for Medical Research (NSW Branch) Scientific Meeting, Sydney, NSW, June 2002.

Additional Info

Recognizes Tropomyosin, 9a containing. Species crossreactivity: Human, rat and mouse.