Tropomyosin

Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan. \n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilution should be determined by the researcher. \n\nPositive Control: \nSkeletal or cardiac muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8664-04

Size

500ul

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Rat, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T8664-04X

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

Additional Info

Recognizes chicken Tropomyosin. Reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha- tropomyosin and skeletal alpha-and beta-tropomyosins. Species Crossreactivity: human and rat.