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Tropomyosin (Tropomyosin beta Chain, Beta-tropomyosin, Tropomyosin-2, TPM2)

Tropomyosin (Tropomyosin beta Chain, Beta-tropomyosin, Tropomyosin-2, TPM2)

Cat no: T8664-04B

Supplier: United States Biological
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The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35-45kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells. Applications: Suitable for use in Western Blot, Immunohistochemistry, immunofluorescence, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (frozen)1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8664-04B
Reactivities: Human, Mouse, Rat, Chicken/Bird
Hosts: Mouse
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes chicken Tropomyosin. Species Crossreactivity: mouse, rat, human.

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