Troponin C, Cardiac (Cardiac Troponin C, CMH7, cTnI, Slow Troponin C1, Troponin C Slow, Troponin C Slow Skeletal and Cardiac Muscles, TN C, TNC, TN-C, TNNC, Troponin C Type 1 (Slow), Troponin C1 Slow, TNNC 1, TNNC1, TNNC1 Protein, Troponin I Type 3 (Cardi

Troponin (Tn) is the central regulatory protein of striated muscle contraction. Tn consists of three components: TnI which is the inhibitor of actomyosin ATPase, TnT which contains the binding site for tropomyosin and TnC. The binding of calcium to TnC abolishes the inhibitory action of Tn on actin filaments, and as a result of calcium ion uptake by troponin C, the elements of the muscle thin filament undergo a series of allosteric changes which allow the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8665-01A

Size

200ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Sarko, J. & Pollack, C.V., Cardiac troponins. J Emerg Med 23:57-65 (2002). 2. Gomes, A.V. et al. . IUBMB Life 54:323-33 (2002). 3. Hamm, C.W., Thromb Res 103 Suppl 1:S63-9 (2001). 4. Labugger, R. et al. E Circulation 102:1221-6 (2000). 5. Wu, A.H. et al. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem 44:1198-208 (1998). 6. Katrukha, A.G. et al. Troponin I is released in bloodstream of patients with acute myocardial infarction not in free form but as complex. Clin Chem 43:1379-85 (1997).

Additional Info

Recognizes human Troponin C at ~19kD.