Troponin C, an 18kD subunit of the Troponin complex found on actin filaments in muscle tissue. Troponin C (TnC) is responsible for binding calcium ions. In the absence of calcium, TnC has an inhibitory action on the actin filaments. When calcium binds to TnC, a conformational change takes place in Troponin I, which in turn causes a change in another associated protein, tropomyosin. Troponin and tropomyosin is repositioned away from the actin filaments, leaving the myosin binding sites available for cross-bridges to form and muscle contraction to occur.
T8665-10B recognizes free cardiac Troponin C and TnC in binary complex with cardiac Troponin I. The antibody does not recognize free cardiac Troponin I or cardiac Troponin T.
T8665-10B may be used as a capture antibody in ELISA assays for human Troponin C in combination with T8665-01E as a detection reagent.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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