Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Cardiac Troponin T, a 36kD protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.\n\nTroponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter

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SPECIFICATIONS

Catalog Number

T8665-15

Size

1mg

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

>90%. Purified by chromatography on protein A sepharose. Purity is tested by electrophoresis.

Isotype

IgG2b

References

General References: \nKr

Additional Info

Reacts with free cardiac Troponon I (cTnl) and cTnl forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between amino acid residues 186 and 192 of cTnI. Crossreactivity with skeletal troponin I from human, rat and mouse is observed.