Troponin I, Cardiac (cTnI)

Human cardiac-specific troponin I (TnI) is an integral inhibitory protein of cardiac muscle which exists as a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin to form a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, enabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. \n\nThe measurement of blood TnI and TnT levels is an important diagnostic indicator of heart muscle damage, and can be used to differentiate between angina and myocardic infarction in patients with chest pain. Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8665-18A

Size

200ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Takeda, S. (2005) Crystal structure of troponin and the molecular mechanism of muscle regulation. J. Electron Microscopy 54 (supplement 1): i35-i41. 2. Jeremias, A. and Gibson, C.M. (2005) Narrative review: Alternative causes for elevated cardiac troponin levels when acute coronary syndromes are excluded. Ann. Intern Med. 142: 786-791.

Additional Info

Recognizes human cardiac-specific troponin I (TnI).