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Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

Troponin I, cardiac muscle, PHOSPHORYLATED (Cardiac Troponin I, TNNI3, TNNC1)

Cat no: T8665-18M

Supplier: United States Biological
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Troponin I is a 24kD subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: For the detection of phosphoproteins, phosphatase inhibitors such as 10mM sodium fluoride and 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8665-18M
Reactivities: Human, Rat, Bovine, Canine, Feline, Goat, Porcine
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Sakthivel, S. et al (2005) In Vivo and in Vitro Analysis of Cardiac Troponin I Phosphorylation. J. Biol. Chem. 280: 703-714. 2. Layland, J. et al (2005) Regulation of cardiac contractile function by troponin I phosphorylation. Cardiovasc. Res. 66: 12-21.
Additional info: Recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I. Species Crossreactivity: rat, feline, bovine, canine, goat and porcine.

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