Troponin T, Cardiac (cTnT) (BSA & Azide Free)

Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.\n\nApplication:\nSuitable for use in Electron Microscopy, Immunofluorescence, and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nCardiac muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8665-23AX

Size

100ug

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, GP, Prc, Rabbit

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See T8665-23A.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Saba Z, etal., Circulation, 1996, 94(3):472-6. 2. Anderson PA. Current Opinion in Cardiology, 1995 Jan, 10(1):33-43. 3. Anderson PA., et al., Circulation Res, 1995, 76(4):681-6. 4. Anderson PA., et al., Circulation Res, 1995, 76(4):681-6. 5. Greig A. et al., Circulation Research, 1994 Jan, 74(1):41-7. 6. McMahon DK., et al., American Journal of Physiology, 1994 Jun, 266(6 Pt 1):C1795-802. 7. Anderson PA., et al., Basic Research in Cardiology, 1992, 87 Suppl 1:117-27. 8. Malouf NN., et al., Journal of Biological Chemistry, 1992 May 5, 267(13):9269-74. 9. Anderson PA., et al.,Circulation Research, 1991, 69(5):1226-33. 10. Nassar R., et al., Circulation Research, 1991, 69:1470-5.

Additional Info

Recognizes the cardiac isoform of Troponin T (cTnT). Species Crossreactivity: Human, porcine, canine, rabbit, rat, mouse, guinea pig, chicken, sparrow and goldfish. Does not react with frog.