Troponin T (TNT, TNTF)

The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle (TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle (TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.\n\nUniProt Number:\nHuman(P13805), Mouse(O88346), Rat(Q7TNB2), Human(P45379), Mouse(P50752), Rat(P50753), Human(P45378), Mouse(Q9QZ47), Rat(P09739)\n\nGene ID:\nTNNT1; TNNT2; TNNT3\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145325

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat, Ch/Bird, Rabbit

Form

Ascites, supplied as a lyophilized powder. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

P Type

Mab

Isotype

IgG1

References

1. Samson, F.; de Jong, P. J.; Trask, B. J.; Koza-Taylor, P.; Speer, M. C.; Potter, T.; Roses, A. D.; Gilbert, J. R. : Assignment of the human slow skeletal troponin T gene to 19q13.4 using somatic cell hybrids and fluorescence in situ hybridization analysis. Genomics 13: 1374-1375, 1992.\n2. Durand, J.-B.; Bachinski, L. L.; Bieling, L. C.; Czernuszewicz, G. Z.; Abchee, A. B.; Yu, Q. T.; Tapscott, T.; Hill, R.; Ifegwu, J.; Marian, A. J.; Brugada, R.; Daiger, S.; Gregoritch, J. M.; Anderson, J. L.; Quinones, M.; Towbin, J. A.; Roberts, R. : Localization of a gene responsible for familial dilated cardiomyopathy to chromosome 1q32. Circulation 92: 3387-3389, 1995.\n3. Mao, C.; Baumgartner, A. P.; Jha, P. K.; Huang, T. H.-M.; Sarkar, S. : Assignment of the human fast skeletal troponin T gene (TNNT3) to chromosome 11p15.5: evidence for the presence of 11pter in a monochromosome 9 somatic cell hybrid in NIGMS mapping panel 2. Genomics 31: 385-388, 1996.

Additional Info

Recognizes rat, rabbit and chicken Troponin T. No cross reactivity with other proteins.