The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle (TNNT1), cardiac muscle (TNNT2),and fast skeletal muscle (TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 2-4ug/ml
Immunohistochemistry (Formalin fixed paraffin, formalin or acetone fixed frozen): 4-8ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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