TRPA1 (Transient Receptor Potential Cation Channel Subfamily A Member 1, Ankyrin-like with Transmembrane Domains Protein 1, ANKTM1, Transformation Sensitive Protein p120)

Transient receptor potential ion channels (TRPCs) are a superfamily of six transmembrane segment-spanning, gated cation channels. TRPA1 is a TRP-related channel that responds to cold temperatures and pungent compounds and plays a role in both nociceptor and hair cell transduction. It is a transformation-associated gene product in lung epithelia, whereas its protein distribution is primarily restricted to sensory neurons. Blocking TRPA1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage. It is now known that TRPA1 protein is also widely expressed outside of the CNS and is dys-regulated during oncogenic transformation.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry (Paraffin): 1:100-1:250\nImmunohistochemistry (Frozen): 0.5-1.0ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman brain membrane fraction\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8666-12C

Size

25ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by peptide immunoaffinity chromatography.

Isotype

IgG

References

1. Yu S, Gao G, Peterson BZ, et al. TRPA1 in mast cell activation-induced long-lasting mechanical hypersensitivity of vagal afferent C-fibers in guinea pig esophagus. Am J Physiol Gastrointest Liver Physiol 2009;297(1):G34-42.

Additional Info

Recognizes human TRPA1 at ~110kD. Species Crossreactivity: mouse. Species Sequence Homology: rat, 69%