TRPC3 (Transient receptor protein 3)

TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.\n\nUniProt Number:\nQ13507\n\nGene ID:\nTRPC3\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145326

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Becker, E. B. E., Oliver, P. L., Glitsch, M. D., Banks, G. T., Achilli, F., Hardy, A., Nolan, P. M., Fisher, E. M. C., Davies, K. E. A point mutation in TRPC3 causes abnormal Purkinje cell development and cerebellar ataxia in moonwalker mice. Proc. Nat. Acad. Sci. 106: 6706-6711, 2009.\n2.Philipp, S., Strauss, B., Hirnet, D., Wissenbach, U., Mery, L., Flockerzi, V., Hoth, M. TRPC3 mediates T-cell receptor-dependent calcium entry in human T-lymphocytes. J. Biol. Chem. 278: 26629-26638, 2003.\n3.van Rossum, D. B., Patterson, R. L., Sharma, S., Barrow, R. K., Kornberg, M., Gill, D. L., Snyder, S. H. Phospholipase C-gamma-1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature 434: 99-104, 2005.\n

Additional Info

Recognizes human, mouse and rat TRPC3. No crossreactivity with other proteins.