TRPM8, ID (TRPM8, LTRPC6, TRPP8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8)

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:50-100\nImmunohistochemistry: 1:50-100\nImmunofluorescence: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

043293

Size

200ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Isotype

IgG

References

Yee, N.S., et al. Cancer Lett. 297(1):49-55(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Kuhn, F.J., et al. J. Biol. Chem. 285(35):26806-26814(2010) Gkika, D., et al. Oncogene 29(32):4611-4616(2010) Van Haute, C., et al. ScientificWorldJournal 10, 1597-1611 (2010) :

Additional Info

Human

Alternative Names

TRPM8, LTRPC6, TRPP8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8