TRPM8, or Transient receptor potential cation channel subfamily M member 8, is a receptor-activated non-selective cation channel involved in the detection of sensations such as coolness, by being activated by cold temperatures below 25 degrees Celsius. It is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Activated upon depolarization, changes in temperature result in graded shifts of voltage-dependent activation curves for TRPM8. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. TRPM8 is expressed in the prostate, and it is also expressed in most prostate tumors.
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Not recommended for use in Flow Cytometry, Immunohistochemistry or Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Positive Controls:
HT-1376, LnCaP and A549 cell lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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