Trypsin, 2X, Bovine Pancreas, Sterile, Irradiated

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors.\n\nQuality Control: \nSDS-PAGE\n\nActivity:\n(same/more than)180u/mg Protein\n(1mg trypsin (same/more than) 180 TAME units, 10,350 BAEE units, 3450 USP/NF units)\n\nUnit Definition:\n1 unit of trypsin hydrolyzes 1umole of p-toluenesulfonyl-L-arginine methyl ester (TAME) per minute @ 25C, pH 8.2, in the presence of 0.01M calcium ion.\n\nVirus Testing: None Detected\nBovine Mycoplasma, Bovine Adenovirus (BAV), Rabies, Bovine Blue Tongue (BTV), Reovirus (REO), Bovine Parvovirus (BPV), Bovine Resp. Synctial (BRSV), Bovine Diarrhea virus.\n\nAppliations:\nSutiable for use in Protein Sequencing, Cell and Tissue Disassociation. Other applications not tested.\n\nRecommended Dilutions:\nMonolayer Dissociation: 0.002-0.01% depending upon strain.\nPrimary Embryo Tissues: 0.01-0.1%\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume,aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: North America\n\nCertificate of Origin\nThe raw animal product used in the manufacturing of Trypsin is derived from materials originating in North America. Material is processed in a USDA inspected facility.\n\nNote:\nTrypsin is subject to a pH (same/less than)3.0 for (same/more than)5 hours during processing.

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