Tryptone is a specially formulated enzymatic digest of casein, similar to Peptone from gelatin. It is used to supply higher growth rates when used in molecular biology media preparations such as YPD Broth and Agar. Used as a nitrogen source in culture media for fastidious and non-fastidious organisms. Also used to differentially select bacteria based on carbohydrate intake since Tryptone is carbohydrate deficient.
Typical Amino Acid Profile: Ala 2.8%, Arg 3.2%, Asp 6.7%, Cys 0.3%, Glu 18%, Gly 1.8%, His 2.4%, Ile 4.7%, Leu 7.7%, Lys 7.0%, Met 2.5%, Phe 4.1%, Pro 9.1%, Ser 5.1%, Thr 3.9%, Trp 1.0%, Tyr 1.3%, Val 6.0%
Appearance: Light tan, homogenous, free flowing powder
Solubility: Light yellow to amber, clear, complete
pH (2%): 6.5-7.5
Ash: (same/less than)15%
Moisture: (same/less than)5%
Amino Nitrogen: (same/more than)4%
Total Nitrogen: (same/more than)10%
Undigested Proteins: Negative
Reducing Sugars:
Negative
Proteases: Trace positive to positive
Nitrites: Negative
Tryptophan: Trace Positive to positive
Growth Supporting Properties:
Agar Formulation, Broth Formulation: Growth Test
Standard Plate Count: (same/less than)5000cfu/g
Molds/ Yeasts: (same/less than)100CFU/g
Coliforms: <10CFU/g
E. coli: Negative
Salmonella: Negative/25g
Certificate of Origin:
The raw animal product used in the manufacturing of Tryptone is derived from casein, a milk product derived from bovine, originating in New Zealand where there have been no reported cases of bovine spongiform encephalopathy (BSE). Tryptone is processed in Mexico. During processing of the product, a batch is heated to a minimum of 80 degrees C for 1 hour including a minimum of 5 minutes at 105 degrees C or greater and then dried at a minimum of 140 degrees C. The enzyme used in processing is porcine-derived from the USA.
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