Home  >  Products  >  Tryptophan Hydroxylase, phosphorylated (Ser260) (TPH, TPRH, MGC119994, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase, Tryptophan 5-monooxygenase 1, Tryptophan Hydroxylase 1, TPH 1, TPH1)
Tryptophan Hydroxylase, phosphorylated (Ser260) (TPH, TPRH, MGC119994, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase, Tryptophan 5-monooxygenase 1, Tryptophan Hydroxylase 1, TPH 1, TPH1)

Tryptophan Hydroxylase, phosphorylated (Ser260) (TPH, TPRH, MGC119994, Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase, Tryptophan 5-monooxygenase 1, Tryptophan Hydroxylase 1, TPH 1, TPH1)

Cat no: T9014-82B

Supplier: United States Biological
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Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T9014-82B
Reactivities: Human, Mouse, Rat, Xenopus/Amphibian
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: 10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017. 2. Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823. 3. Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.
Additional info: Specific for the ~55k Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus. Species Crossreactivity: Human, mouse, Xenopus, and rat based on 100% antigen sequence homology.

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