Home  >  Products  >  Tryptophan Hydroxylase, phosphorylated (Ser58) (TPH, TPRH, TRPH, Tryptophan Hydroxylase 1, Tryptophan 5-hydroxylase 1, TPH1, MGC119994, Tryptophan 5-monooxygenase 1)
Tryptophan Hydroxylase, phosphorylated (Ser58) (TPH, TPRH, TRPH, Tryptophan Hydroxylase 1, Tryptophan 5-hydroxylase 1, TPH1, MGC119994, Tryptophan 5-monooxygenase 1)

Tryptophan Hydroxylase, phosphorylated (Ser58) (TPH, TPRH, TRPH, Tryptophan Hydroxylase 1, Tryptophan 5-hydroxylase 1, TPH1, MGC119994, Tryptophan 5-monooxygenase 1)

Cat no: T9014-82E

Supplier: United States Biological
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Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Human dorsal and raphe nucleus Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9014-82E
Reactivities: Human, Mouse, Rat, Xenopus/Amphibian
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES (pH 7.5), 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=1.14.16.4
References: 1. Martinez A, Knappskog, PM. and Haavik J. 2001. A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr. Med. Chem., 8:1077-1091. 2. Jiang GC, Yohrling GJ., Schmitt JD. and Vrana K.E. 2000. Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J. Mol. Biol. 302:1005-1017. 3. Banik U, Wang GA, Wagner, PD, and Kaufman S. 1997. Interaction of phosphorylated tryptophan hydroxylase with 14-3-3 proteins, J. Biol. Chem. 272:26219
Additional info: Recognizes human Tryptophan Hydroxylase, phosphorylated at (Ser58). Species crossreactivity: mouse, rat, and xenopus.

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