Tryptophan Hydroxylase (TPH) belongs to the biopterin-dependent aromatic amino acid hydroxylase family. This enzyme catalyzes the first and rate limiting step in the biosynthesis of serotonin. Mutations in this gene have been implicated in a variety of disorders including depression, aggression and schizophrenia.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-1ug
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.