TSC, Rat Control Peptide, (Thiazide-sensitive Na Cl Transporter, NCC)

Control Peptide for T9100-10 (antiserum) and T9100 (affinity purified)\n\nChloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nAntibody Blocking: 5-10ug per 1ul T9100-10 (antiserum) or per 1ug T9100-10A (affinity purified antibody).\nELISA: 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9100-12

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% sodium azide, 40% glycerol.

Purity

Highly purified

References

(1). Gamba G et al (1994) J Biol. Chem. 269, 17713-17722; Simon DB et al (1996) Nature Genet. 12, 24-30; Mount DB et al (1995) J Am. Soc. Nephrol. 6, 347; Igarashi P et al (1995) Am. J. Physiol. 269, F405-418; Haas M et al (1987) Am. J. Physiol. 253, C243; Mount DB et al (1998) J. Exptl. Biol. 201, 2091 (review).

Additional Info

Recognizes rat Thiazide-Sensitive Na-Cl Transporter (TSC, NCC). The immunogen shows no significant sequence homology with other CCC. Species sequence homology: mouse and rabbit: 98% and human 83%.