TSC (Thiazide-sensitive Na Cl Transporter, NCC)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. \nThree groups of proteins have been identified in CCC family based upon their structures, ligands, and inhibitors. These are the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+-Cl--cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.\nIn the distal convoluted tubule (DCT) of the kidney, the primary apical entry pathway for Na is via the thiazide-sensitive Na-Cl transporter (TSC or NCC; human 1021 aa, rat/mouse 1002 aa; ~60 % identity with NKCC). TSC is specifically detected at the apical cell membrane of DCT.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 using ECL.\nELISA: 1:10,000-50,000. T9100-12 Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9101-10

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1.. Gamba G et al (1994) J Biol. Chem. 269, 17713-17722; 2. Simon DB et al (1996) Nature Genet. 12, 24-30; 3. Mount DB et al (1995) J Am. Soc. Nephrol. 6, 347; 4. Igarashi P et al (1995) Am. J. Physiol. 269, F405-418; 5. Haas M et al (1987) Am. J. Physiol. 253, C243; 6. Mount DB et al (1998) J. Exptl. Biol. 201, 2091 (review).

Additional Info

Recognizes rat Thiazide-Sensitive Na-Cl Transporter (TSC, NCC). The immunogen shows no significant sequence homology with other CCC. Species sequence homology: mouse and rabbit: 98% and human 83%.