TSC2 (Tuberous Sclerosis Complex 2, Tuberous Sclerosis 2 Protein, FLJ43106, LAM, TSC4, Tuberin)

Tuberous sclerosis complexe-2 (TCS2 also known as Tuberin) is a tumor suppressor that form a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:50\nWestern Blot: 1:200-1:1000 (180kD)\nImmunohistochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

T9100-25A

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.05% BSA, 0.01% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Lamb,et al. Nature Cell Biol. 2: 281-287 2. Xiao et al J. Biol. Chem. 272: 6097-6100 3. Manning, B. D.; Tee, A. R.; Logsdon, M. N.; Blenis, J.; Cantley, L. C. : Molec. Cell 10: 151-162, 2002. 4. Shumway, S. D.; Li, Y.; Xiong, Y. : J. Biol. Chem. 278: 2089-2092, 2003. 5. Inoki, K.; Li, Y.; Zhu, T.; Wu, J.; Guan, K.-L. : 4: 648-657, 2002.

Additional Info

Recognizes human TSC2. Predicted to detect isoform 2-4 based on sequence homology. Species Crossreactivity: mouse and rat.