Tuberin, phosphorylated (Ser1387) (Tuberous Sclerosis 2 Protein, TSC2, FLJ43106, LAM, TSC4)

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571\nregulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and eventual inhibition of translation (3,6,7). Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis (8). Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling (9).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1%\nTween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9170-06P

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.(2) Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119. (3) Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.\n(4) Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021. (5) Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370. (6) Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.\n(7) Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657. (8) Inoki, K. et al. (2003) Cell 115, 577-590.\n(9) Inoki, K. et al. (2006) Cell 126, 955-968.

Additional Info

Recognizes endogenous levels of human tuberinonly when phosphorylated at (Ser1387). Species Crossreactivity: mouse, monkey and rat.