Home  >  Products  >  Tuberin, phosphorylated (Ser1387) (Tuberous Sclerosis 2 Protein, TSC2, FLJ43106, LAM, TSC4)
Tuberin, phosphorylated (Ser1387) (Tuberous Sclerosis 2 Protein, TSC2, FLJ43106, LAM, TSC4)

Tuberin, phosphorylated (Ser1387) (Tuberous Sclerosis 2 Protein, TSC2, FLJ43106, LAM, TSC4)

Cat no: T9170-06P

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and eventual inhibition of translation (3,6,7). Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis (8). Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling (9). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9170-06P
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: (1) Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.(2) Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119. (3) Manning, B. D. et al. (2002) Mol. Cell 10, 151-161. (4) Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021. (5) Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370. (6) Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967. (7) Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657. (8) Inoki, K. et al. (2003) Cell 115, 577-590. (9) Inoki, K. et al. (2006) Cell 126, 955-968.
Additional info: Recognizes endogenous levels of human tuberinonly when phosphorylated at (Ser1387). Species Crossreactivity: mouse, monkey and rat.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave