Home  >  Products  >  Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Cat no: T9154-01C

Supplier: United States Biological
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The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates alpha-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remod-eling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3). Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:800 Immunoprecipitation: 1:50 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9154-01C
Reactivities: Human, Mouse, Rat, Non-Human Primate, Zebrafish/Fish
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 40ul
Form: Supplied in liquid 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Westermann, S. & Weber, K., Nat. Rev. Mol. Cell Biol. 4: 938-947 (2003). 2. Creppe, C., et al., Cell 136: 551-564 (2009). 3. Hubbert, C., et al., Nature 417: 455-458 (2002).
Additional info: Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey, zebrafish.

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