Tubulin, alpha, acetylated (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. Stable microtubules (MTs) are known to contain acetylated alpha-tubulin. Acetylated tubulin is found in the dense networks and bundles of microtubules in the sensory and supporting cells. MTs containing acetylated alpha-tubulin in Ni2+-treated cells were resistant to disassembly induced by nocodazole, and at least partially resistant to cold temperature (0 degrees Celsius), which also depolymerizes MTs. Since acetylated alpha tubulin serves as a marker for the presence of stable MTs, the marked enhancement of alpha tubulin acetylation in Ni2+-treated cells indicates that stabilization of MTs may be an important mechanism by which Ni2+ induces cell injury since stabilized MTs in turn should favor MT bundling. The acetylated microtubules have been shown to be more drug resistant than nonacetylated microtubules. This increased stability is most likely due to the acetylation process.\n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot (Human, mouse, rat): 1-3ug/ml\nImmunofluorescence (Human, mouse): 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nSource: Ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9154-10

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG2b

References

1. Shi Q, et al. Curr Pharm Des 4(3): 219-248,(1998). 2. Li W, et al. Toxicol Appl Pharmacol 140(2): 461-470, (1996). 3. Ogata Y, Slepecky NB. Hear Res 86(1-2): 125-131, (1995). 4. Thyberg J, Mosalewski S. Cel Tissue Res 273(3): 457-466, (1993). 5. LeDizet M, Piperno G. J Cell Biol 103(1): 13-22, (1986). 6. Caron JM, et al. Nature 317(6038): 648-651, (1985). 7. Piperno G, Fuller MT. J Cell Biol 101(6):2085-2094, (1985). 8. Piperno G, et al. J Cell Biol 104(2):289-302, (1987).

Additional Info

Recognizes the ~55kD acetylated form of alpha-tubulin. \nSpecies Crossreactivity: human, mouse and rat. Reactivity was confirmed with NIH3T3 and HeLa cells.