Tubulin, alpha, acetylated (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100kD) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55kD each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.\n\nApplications: \nSuitable for use in ELISA, Immunofluorescence, Western Blot and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000 The dilution was determined using bovine or rat brain preparation. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9154-06L

Size

200ul

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, NHP, Prc, Xen/Amph

Form

Supplied as a liquid in 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

1. LeDizet M & Piperno G Detection of acetylated alpha-tubulin by specific antibodies. Methods Enzymol 196:264-74 (1991). <PUBMED:2034123> 2. Piperno G et al. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J Cell Biol 104:289-302 (1987). <PUBMED:2879846> 3. LeDizet M & Piperno G Identification of an acetylation site of Chlamydomonas alpha-tubulin. Proc Natl Acad Sci U S A 84:5720-4 (1987). <PUBMED:2441392> 4. Piperno G & Fuller MT Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms. J Cell Biol 101:2085-94 (1985). <PUBMED:2415535>

Additional Info

Recognizes tubulin when actylated at Lys40. Species Crossreactivity: bovine, chicken, human, mouse, plant, porcine, primate, rat, Xenopus