Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

There are three classes of the ~50kD tubulin protein: alpha, beta and gamma. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP; however, only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.\n\nApplications: \nSuitable for use in ELISA, Western Blot, screening of expression libraries, identification of tubulin on fixed, frozen tissue sections and staining of microtubular structures in fixed cells by Immunofluorescence microscopy and by light microscopy using PAP complex. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunofluorescence: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9154-05

Size

100ug (200ul)

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified from ascites by sequential precipitation with caprylic acid and ammonium sulfate solution.

Isotype

IgG1

References

1. Viklicky, V., et al., Cell Biol. Int. Rep. 6: 725 (1982). 2. Draber, P., et al., Eur. J. Cell. Biol. 41: 82 (1986). 3. Draber, P., et al., J. Cell Sci. 92: 519 (1989). 4. Grimm, M., et al., Biochem. Biophys. Acta 914: 83 (1987).

Additional Info

Recognizes alpha tubulin of various species. Recognizes a N-terminal structural domain of alpha tubulin. Reacts with peptides that correspond to position alpha (65-79) of Tubulin; band at 50kD.