Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Fusion Partners: Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.\n\nApplications:\nImmunohistochemistry (frozen)\nELISA\nImmunoprecipitation\nWestern Blot \nRadioimmunoassay \nOptimal working dilutions to be determined by researcher.

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SPECIFICATIONS

Catalog Number

T9154-05D

Size

500ug

Applications

ELISA, IHC, IP, WB

Hosts

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Kilmartin, J.V. et al. (1982). Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. J. Cell. Biol. 93: 576-582.2. Skinner, R.H. et al. (1991). Use of the Glu-Glu-Phe C-terminal epitop

Additional Info

Recognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some crossreactivity with other proteins in certain circumstances, including E. Coli rec A and oxidized actin. Species Crossreactivity: 3H3087 reacts with tubulin from yeast, avian and mammalian.