Alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55kD. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene,IL-alpha-T1. B-alpha-1 encodes a predicted 451aa protein that is 100% identical to the rat homolog and differs by only 2 and 3aa from the pig and chicken homologs, respectively.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.