Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (Cy3)

Applications: \nSuitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Cultured chicken fibroblasts or BHK cells.\n\nCellular Localization: Major constituent of microtubules.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9155-01L

Size

100ul

Applications

ICC, IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Rabbit

Form

15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4

P Type

Mab

Purity

As reported

Isotype

IgG1

References

Southwick PL et al. Cyanine dye labeling reagents--carboxymethylindocyanine succinimidyl esters. Cytometry 11:418-30 (1990). <PUBMED:2340776>Matthes T et al. Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule. J Immunol 141:3135-41 (1988). <PUBMED:2459243>Gozes I & Barnstable CJ Monoclonal antibodies that recognize discrete forms of tubulin. Proc Natl Acad Sci U S A 79:2579-83 (1982). <PUBMED:6178107>

Additional Info

It reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain beta-tubulin isoform-related specificity Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine, Hamster, Frog, Moth, Wheat and Rabbit.