Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1:200 for 30 minutes. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.\nOptimal dilutions to be determined by the researcher\n\nPositive Control: \nMAD109 cells, MCF-7 cells; Skin or lung\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9154-05G

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, GP, Prc, Yeast/Fun

Form

Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes human Tubulin beta. Blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Does not cause the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Species Crossreactivity: Bovine, Porcine, Rat, Mouse, Guinea pig, Gerbil, Frog, Chicken, Yeast, Fungi, Sea urchin, Myxamoeba, and Plasmodium.