Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. The effects on tubulin messenger RNA levels and tubulin protein synthesis when treating cells with microtubule-depolymerizing drugs or when directly microinjecting cells with tubulin suggest that non-polymerized tubulin depresses its own synthesis. Accumulation of tubulin protein and an increased array of microtubules have been associated with contractile dysfunction in cardiac myocytes after pressure overload in vivo. Studies have also shown that cardiac activity can increase the amount of beta-tubulin in rat cardiac myocytes. Tubulin production in cultured cardiac myocytes can be regulated directly by mechanical forces. In mechanically challenged hearts, the accumulation of beta-tubulin and the development of contractile dysfunction may be directly related to the mechanical forces imposed on the myocardium during the onset and progression of cardiovascular disease.\n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot (Human, mouse and rat): 1-3ug/ml\nImmunofluorescence (C. elegans): 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse NIH3T3 fibroblast cells, rat brain, and mouse testis.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9155-01A

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG1,k

References

1. Shi Q, et al. Curr Pharm Des 4(3):219-248,(1998). 2. Watson PA, et al. Am J Physiol 271(2 pt 1):C684-C689, (1996). 3. Buttgereit D, et al. Int J Dev Biol 40(1):189-196,(1996). 4. Caron JM, et al. Nature 317(6038):648-651, (1985). 5. Siddiqui SS, et al. J Neurosci 9(8):2963-2972, (1989).

Additional Info

Recogizes human b-tubulin at ~50kD. Shown to bind to the two major and one of the minor b-tubulin isotypes. Species Crossreactivity: mouse, rat, and C. elegans.