Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Applications: \nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Cultured human fibroblasts.\n\nCellular Localization: Major constituent of microtubules.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9155-01H

Size

100ul

Applications

ICC, IF, WB

Hosts

Mouse

Reactivities

Hum, Rat, Bov, Ch/Bird

Form

0.1% Sodium Azide; Raw Ascites

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). <PUBMED:2194680>Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). <PUBMED:2404018>Siddiqui SS et al. Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans. J Neurosci 9:2963-72 (1989). <PUBMED:2475594>Piperno G et al. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J Cell Biol 104:289-302 (1987). <PUBMED:2879846>

Additional Info

Recognizes the two major beta tubulin isotypes and one of the minor beta-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes, to neuronal axons of the larva of Ciona intestinalis (ascidian), and to tubulin of chicken and mammals (e.g., cultured human fibroblasts, bovine, and rat brain tissue). In C. elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels, and found reactive with heat-denatured and reduced mammalian preparations in immunoblotting. Species Crossreactivity: Crossreacts with all Mammals, Chicken and Sea Urchin.