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Tubulin, beta (BSA & Azide Free) (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Cat no: T9155-12X

Tubulin, beta (BSA & Azide Free) (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. \n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml for 2 hours RT.\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLs174T and MAD109 cells, Skin or lung. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9155-12X

Size

100ul

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T9155-12.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG3

References

1. Gozes, I. and Barnstable, C.J., Proc. Natl. Acad. Sci. USA 79: 2579 (1982). 2. Matthes, T., et al., J. Immunol. 141: 3135 (1988).

Additional Info

Recognizes human Tubulin, beta at ~55kD. \nSpecies Crossreactivity: bovine, rabbit, mouse, chicken, rat, frog, moth and wheat.

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