Tubulin, beta I (Tubulin beta 1, TUBB 1, TUBB1)

Applications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Cultured chicken fibroblasts.\n\nCellular Localization: Major constituent of microtubules.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9155-02D

Size

100ul

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Xen/Amph

Form

Raw Ascites

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). <PUBMED:9580378>Oakley BR Gamma-tubulin: the microtubule organizer? Trends Cell Biol 2:1-5 (1992). <PUBMED:14731630>Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). <PUBMED:2194680>Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990). <PUBMED:2404018>

Additional Info

No reactivity with other tubulin isotypes is observed. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, Xenopus laevis, bovine, Chicken, canine and Hamster.