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Tubulin, beta I (Tubulin beta 1, TUBB 1, TUBB1)

Tubulin, beta I (Tubulin beta 1, TUBB 1, TUBB1)

Cat no: T9155-02D

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Cultured chicken fibroblasts. Cellular Localization: Major constituent of microtubules. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T9155-02D
Reactivities: Human, Mouse, Rat, Bovine, Canine, Chicken/Bird, Xenopus/Amphibian
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Raw Ascites
P type: Mab
Isotype: IgG1
Purity: Ascites
References: Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). Oakley BR Gamma-tubulin: the microtubule organizer? Trends Cell Biol 2:1-5 (1992). Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990).
Additional info: No reactivity with other tubulin isotypes is observed. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, Xenopus laevis, bovine, Chicken, canine and Hamster.

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