Tubulin, beta IV (Tubulin beta 4, TUBB 4, TUBB4, Beta-4, Beta-4 Tubulin, MC1R, Tubulin beta 3)

The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin exists in all cells as a 100kD protein, a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. Both alpha and beta tubulins consist of various isotypes. For beta tubulin, six evolutionarily conserved isotypes were identified (designated beta I-beta VI). Beta IV exists as two subtypes in mammals, differing from each other at 10 positions. beta IVa is brain specific, whereas beta IVb is ubiquitous, and both appear to be constitutive. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: paraffin sections\nWestern Blot: 1:400 detects a band at ~55kD and possibly the 100kD dimer.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9155-25

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird

Form

Supplied as a liquid, 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1.Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). <PUBMED:9580378>Renthal R et al. Beta IV is the major beta-tubulin isotype in bovine cilia. Cell Motil Cytoskeleton 25:19-29 (1993). <PUBMED:8519065>Banerjee A et al. Preparation of a monoclonal antibody specific for the class IV isotype of beta-tubulin. Purification and assembly of alpha beta II, alpha beta III, and alpha beta IV tubulin dimers from bovine brain. J Biol Chem 267:5625-30 (1992). <PUBMED:1544937> Banerjee A & Luduena RF Kinetics of colchicine binding to purified beta-tubulin isotypes from bovine brain. J Biol Chem 267:13335-9 (1992). <PUBMED:1618835>

Additional Info

Recognizes human Tubulin, beta IV. Does not react with other tubulin isotypes. Species Crossreactivity: mouse, rat, hamster, bovine and chicken